Figure 2.

Normal and keloid ESS in vivo. (A–D) Shown are histological sections of normal (A, B) and keloid (C, D) ESS 12 weeks after transplantation to mice. Sections in the top panels (A, C) were stained with Tango stain, similar to hematoxylin; bottom panels (B, D) show Masson's trichrome-stained sections. Note the densely packed disorganized collagen fibers in the keloid ESS. Scale bars for all sections, 200 μm. (E) Quantitation of the total thickness of the grafted tissue demonstrated that ESS prepared with keloid cells were significantly thicker than ESS prepared using normal cells. ESS, engineered skin substitutes. Color images are available online.