Table 3. Primer Sequences of Target Genes Used for Real-Time PCR of Gastric Mucosa Samples.
gene | forward primer (5′ to 3′) | reverse primer (5′ to 3′) | product length (bp) | Tm (°C) |
---|---|---|---|---|
β-actin | CATCACCAACTGGGACGACA | GTTGGCCTTAGGGTTCAGGG | 121 | 55 |
ghrelin | AGTGCAGCAGAGAAAGGAGTC | GATCCCAACATCACAGGGGG | 151 | 55 |
MBOAT4 | GGATCCCAGGCACTCTCTCT | TGCCGACAATCAGTCAATCCA | 123 | 59 |
RETN | GCTCTCTCCCTCCTCTTCCT | CGACATCCCGGATCTTCTCATT | 100 | 55 |
GCG | GCGAGATTTCCCAGAGGAAGTT | AAAGTCTCGGGTGGCAAGATT | 115 | 55 |
GCGR | CGTGCAGAGCTGGTCTGTAATA | CGCCGTGGCTACCTTTGT | 127 | 55 |
SST | TGCTCTCTGAACCCAACCAG | CAGCCAGCTTTGCGTTCTC | 148 | 55 |