Skip to main content
. 2019 Nov 23;2019:5820839. doi: 10.1155/2019/5820839

Figure 4.

Figure 4

Rab5c- and EEA1-dependent transportation of KCa2.3 and KCa3.1 proteins. (a, b) Levels of EEA1 (a) or Rab5c (b) in ECs treated with NNP or NP serum. (c) PE serum reduced KCa3.1 levels and enhanced Rab5c and EEA1 levels. (d) LPC increased EEA1 and Rab5c levels in a concentration-dependent manner. (e) Coimmunoprecipitation showing the interaction between Rab5c and KCa3.1 in ECs treated with LPC. Input is lysate without primary Ab. (f, g) Treatment with siRNA against EEA1 recovered KCa2.3 (f) or KCa3.1 (g) levels in ECs treated with PE serum or LPC. Blots shown are representative of three to four experiments performed with three to four different cultures. Bar graphs represent the mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.