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Effect of Nnat knockdown on APN suppressing mRNA expression of inflammatory factors. Relative mRNA expression levels of (a) MCP-1, (b) IL-6, (c) IL-8, and (d) TNF-α were detected using qPCR. Data are presented as the mean ± standard error of the mean for six independent experiments. ∗p < 0.05 vs. the control group; ▲p < 0.05 vs. the LPS group; #p < 0.05 vs. the LPS+Ad-apM1 group; &p < 0.05 vs. the LPS+Ad-apM1+Ad-nnat-shRNA group.
