KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| DH5α E.coli Competent Cells | New England Biolabs | C2987H |
| pM2rtTA lentivirus | Gift from Donald Kohn’s lab | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Doxycycline hyclate | Sigma-Aldrich | D9891 |
| Blasticidin S-HCl | Thermo Fisher Scientific | A1113902 |
| Puromycin Dihydrochloride | Thermo Fisher Scientific | A1113802 |
| Nonanedioic Acid | Sigma-Aldrich | 246379 |
| 2-coumaranone | Sigma-Aldrich | 124591 |
| Decanoic Acid | Sigma-Aldrich | C1875 |
| Acetophenone | Sigma-Aldrich | A10701 |
| Prenyl Acetate | Sigma-Aldrich | W420201 |
| Vanilic Acid | Sigma-Aldrich | H36001 |
| Forskolin | Sigma-Aldrich | F3917 |
| Critical Commercial Assays | ||
| Dual-Glo Luciferase Assay System | Promega | E2920 |
| White Poly-D-Lysine Coated 96-well Plates | VWR | 89092–710 |
| Lipofectamine 2000 | Thermo-Fisher Scientific | 11668027 |
| CD293 Medium | Thermo-Fisher Scientific | 11913019 |
| OptiMEM Medium | Thermo-Fisher Scientific | 31985062 |
| Qiashredder Tissue and Cell Homogenizer | Qiagen | 79654 |
| RNEasy MiniPrep Kit | Qiagen | 74104 |
| Superscript IV | Thermo-Fisher Scientific | 18090010 |
| HiFI MasterMix | KAPA Biosystems | KR0370 |
| ZymoClean Gel DNA Recovery Kit | Zymo Research | D4001T |
| Gibson Assembly HiFI Mastermix | SGI-DNA | Quote Needed |
| MluI Restriction Enzyme | New England Biolabs | R3198 |
| AgeI Restriction Enzyme | New England Biolabs | R3552 |
| T4 DNA ligase | New England Biolabs | M0202S |
| DNA Clean and Concentrator Kir | Zymo Research | D4013 |
| Zyppy-96 Plasmid Miniprep Kit | Zymo Research | D4041 |
| Lipofectamine 3000 | Thermo Fisher Scientific | L3000008 |
| Quick-gDNA Miniprep kit | Zymo Research | D4074 |
| SYBR FAST qPCR Master Mix | Kapa Biosystems | KR0389 |
| Cells-to-cDNA II Lysis Buffer | Thermo Fisher Scientific | AM8723 |
| DNase I | New England Biolabs | M0303S |
| dNTPs | New England Biolabs | N0447 |
| M-MuLV Reverse Transcriptase | Enzymatics | P7040 |
| Rnase Inhibitor | Enzymatics | Y9240 |
| NEB-Next Q5 Mastermix | New England Biolabs | M0544 |
| Experimental Models: Cell Lines | ||
| Human: 293T Cells | ATCC | CRL-3216 |
| Oligonucleotides | ||
| Full list of primers is presented in Table S4 | ||
| Recombinant DNA | ||
| pCRE-Luc | Stratagene | 219076 |
| pRL-SV40 | Promega | E2231 |
| pCI | Promega | E1731 |
| Super PiggyBac Transposase | Systems Bioscience | PB210PA-1 |
| Software and Algorithms | ||
| sklearn | Pedregosa et al., 2011 | http://scikit-learn.org |
| EdgeR | Robinson et al., 2010 | https://bioconductor.org/packages/release/bioc/html/edgeR.html |