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. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Nature. 2019 Oct 21;576(7785):149–157. doi: 10.1038/s41586-019-1711-4

Figure 3. PE3 and PE3b systems nick the non-edited strand to increase prime editing efficiency.

Figure 3.

(a) Overview of prime editing by PE3. After initial synthesis of the edited strand, 5’ flap excision leaves behind a DNA heteroduplex containing one edited strand and one non-edited strand. Mismatch repair resolves the heteroduplex to give either edited or non-edited products. Nicking the non-edited strand favors repair of that strand, resulting in preferential generation of duplex DNA containing the desired edit. (b) The effect of complementary strand nicking on prime editing efficiency and indel formation. “None” refers to PE2 controls, which do not nick the complementary strand. (c) Comparison of editing efficiencies with PE2, PE3, and PE3b (edit-specific complementary strand nick). Editing efficiencies reflect sequencing reads that contain the intended edit and do not contain indels among all treated cells, with no sorting. Values and error bars reflect mean±s.d. of n=3 independent biological replicates.