Table I:
Fluorescence Lifetimes and Oxygen Quenching Constants for TNS-Labeled Lipid Vesicles
| lipid | temp (°C) | τ0 (ns)a | KD × 103 (psi−1) | KS × 103 (psi−1)b |
|---|---|---|---|---|
| DOPC | 5 | 7.1 | 4.02 | 0.28 |
| 15 | 5.9 | 4.28 | 0.31 | |
| 25 | 4.7 | 5.10 | 0.13 | |
| 35 | 3.9 | 4.73 | 0.35 | |
| 45 | 3.1 | 4.56 | 0.18 | |
| DMPC | 5 | 7.8 | 3.69 | 0.29 |
| 15 | 7.6 | 4.97 | 0.28 | |
| 25 | 6.6 | 7.74 | 0.22 | |
| 35 | 4.9 | 7.75 | 0.20 | |
| 45 | 3.9 | 7.88 | 0.11 | |
| DMPC/Chol (6:1) | 5 | 9.0 | 2.51 | 0.54 |
| 15 | 8.6 | 6.14 | 0.29 | |
| 25 | 7.4 | 7.08 | 0.18 | |
| 35 | 4.9 | 8.11 | 0.09 | |
| 45 | 3.9 | 8.74 | 0.13 | |
| DMPC/Chol (3:1) | 5 | 9.2 | 2.64 | 0.70 |
| 15 | 8.1 | 4.22 | 0.47 | |
| 25 | 6.7 | 7.60 | 0.34 | |
| 35 | 5.5 | 6.96 | 0.46 | |
| 45 | 4.0 | 5.87 | 0.72 | |
| DPPC | 5 | 7.5 | 2.54 | 0.23 |
| 17 | 7.1 | 3.34 | 0.36 | |
| 27 | 6.5 | 2.65 | 0.47 | |
| 37 | 5.5 | 4.34 | 0.65 | |
| 45 | 4.6 | 6.36 | 0.19 | |
| 50 | 4.0 | 6.19 | 0.33 | |
| 60 | 3.1 | 6.19 | 0.07 | |
| DPPC-E | 25 | 5.2 | 3.43 | 0.24 |
| 37 | 4.8 | 4.43 | 0.23 | |
| 45 | 3.8 | 7.62 | 0.05 | |
| 55 | 2.6 | 8.25 | 0.00 | |
| 61 | 1.9 | 9.41 | −0.29 |
a
The average of the 10- and 30-MHz phase lifetimes is indicated.
b
These apparent KS values do not prove the existence of a ground-state complex between the fluorophore and quencher (Lakowicz &Weber, 1973).