Fig. 5.

Comparison of alternative splicing in different barley tissues with HR RT-PCR and RNA-seq data. Splicing proportions of four different genes in 5 different barley tissues are presented. a. Hv110; HORVU5Hr1G027080, b. Hv118; HORVU1Hr1G078110, c. Hv173; HORVU7Hr1G062930, d. Hv217; HORVU7Hr1G071060. Schematic transcript/AS models are presented above histograms of PSIs derived from HR-RT-PCR (black) and RNA-seq (white) with standard error bars across three biological repeats. White boxes - exons, lines - introns; chevrons – splicing events; grey boxes region between alternative splice sites; thick intron line represents an intron retention