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. 2019 Dec 11;25:54. doi: 10.1186/s10020-019-0122-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The apoptosis and the target gene of miR-320 were determined. a: The miR-320 expression was determined by qRT-PCR. b: The apoptosis was detected by flow cytometry. c: The apoptosis was up-regulated after inhibited miR-320 expressions. d: The Bcl-2, cytochrome c, C caspase-9 and C caspase-3 expressions were detected by Western blotting. e: After the inhibition of miR-320 expression, the expressions of cytochrome c, C caspase-9 and C caspase-3 were increased and the expression of Bcl-2 was decreased. f: The miR-320 potential target gene was predicted by Targetscan 7.2. g: The PTEN expression was detected by Western blotting. h: The PTEN expression in antago group was up-regulated. ^**P < 0.01 vs. ED, ^##P < 0.01 vs. Scrambled