Fig. 6.

Neither SGLT2 inhibitors nor β-OHB directly alter MC38 tumor cell division. a Impact of SGLT2 inhibitors and insulin on MC38 cell glucose uptake in vitro. In panels a–d, n = 4 in vitro replicates, with groups compared by ANOVA with Bonferroni’s multiple comparisons test, in which each group was compared to the vehicle-treated cells (**P < 0.01, ***P < 0.001, ****P < 0.0001). b V_PDH/V_CS. c Cell division. d Impact of β-OHB and insulin on cell division. e Plasma β-OHB concentrations after 4 weeks of β-OHB supplementation in drinking water, which was removed 2 h before plasma was obtained. f Tumor size. In panels e and f, n = 8 per group, with groups compared by the two-tailed unpaired Student’s t test. All data are presented as the mean ± S.E.M