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. 2019 Dec 11;21:141. doi: 10.1186/s13058-019-1233-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Normal-derived intact organoids in culture resemble in situ normal breast biopsies. Representative fluorescence micrographs of cryostat sections from normal breast biopsies (top) and normal breast organoids (bottom) stained for either K19-AF568, K14-AF488, and DAPI (left) or Muc1-AF568, integrin β4-AF488, and DAPI (right). Note that organoids in the culture retain a double-layered organization