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. 2019 Dec 11;21:141. doi: 10.1186/s13058-019-1233-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — K14⁺/ITGB4⁺ cells dominate in tumor-derived organoid cultures after passaging. Representative fluorescence micrographs of cryostat sections from tumor-derived organoids in passage 2 or 3 stained for K19-AF568/K14-AF488 (upper left), Muc1-AF568/integrin β4-AF488 (upper right), K5-AF488 (lower left), and K17-AF488 (lower right). The nuclei were stained with DAPI. Scale bar, 50 μm