Fig. 1.

The natural history of Fcgr2b^−/− spontaneous LN mice. a, b The temporal levels of serum anti-nuclear antibody (ANA) and anti-double strand DNA (ds-DNA). c Urinary albumin/creatinine ratio (ACR) determined by ELISA in Fcgr2b^−/− mice from age 16 weeks to 40 weeks. d PAS and Masson staining of paraffin-embedded kidney sections taken from wild type (WT) and Fcgr2b^−/− mice at age 32 weeks and 40 weeks. Representative lobular proliferation including the increase of mesangial cells and the expansion of matrix (white arrow), endocapillary proliferation (yellow arrow), adhesion of capillary tufts with Bowman’s capsule (red arrow), and some detachment and vacuolization of podocytes. Immunofluoscent staining of C1q in frozen kidney tissue of wild type (WT) and Fcgr2b^−/− mice at age 32 weeks (moderately positive) and 40 weeks (fully positive). The semi-quantification of kidney injury was determined by respective scoring system. e The temporal levels of renal miR-150 assayed by qPCR in Fcgr2b^−/− mice and WT mice. Data are expressed as mean ± SD from six mice per group. Statistical significance was determined using two-way ANOVA (^#p < 0.05, Fcgr2b^−/− vs. WT; *p < 0.05 each age of Fcgr2b^−/− vs. age 16 week)