Fig. 4.

The effect of LNA-anti-miR-150 on renal pro-fibrotic genes and proteins in LN mice. a Renal mRNA levels of Tgfβ1, αSma, and Fn determined by qPCR; b renal protein levels of TGF-β1, α-SMA, and FN examined by western blotting and the density of the blots; and c immunoflurescent staining of TGF-β1 (red), α-SMA (green), and FN (red) in glomeruli (magnification × 400, bar = 30 μm) and the outer medulla (magnification × 200, bar = 100 μm) of paraffin-embedded kidney sections taken from WT and LN mice treated with the scrambled LNA or LNA-anti-miR-150 (subcutaneous injection twice weekly for 8 weeks). White asterisks represent vessels as positive control of α-SMA. Data are expressed as mean ± SD from six mice per group. Statistical significance was determined using two-way ANOVA (^#p < 0.05, LN vs. WT; *p < 0.05, LNA-anti-miR-150 vs. the scrambled LNA)