Fig. 3.

miR-542-3p targeted BMP7. a After 7 days of induction by β-GP, the protein expression of BMP7 was detected by Western blot. yVSMCs overexpressing miR-542-3p (miR-542-3p) expressed an obviously lower level of BMP7 than control yVSMCs and yVSMCs infected with an empty virus vector. b Quantification of BMP7 expression by densitometric analysis. The relative expression of BMP7 was normalized to that of GADPH. n = 3. Versus control and vector group, **p < 0.01. c BMP7 3′-UTR constructs used in luciferase assay. d Effect of miR-542-3p overexpression on a dual luciferase reporter plasmid containing the BMP7 3′-UTR was analyzed. HEK293T cells were co-transfected with either the BMP7 WT or BMP7 MUT or an empty vector and miR-542-3p (miR-542-3p). Renilla luciferase activity was quantified and normalized to firefly luciferase activity. n = 3. Versus vector group, **p < 0.01