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. 2019 Dec 11;13:67. doi: 10.1186/s40246-019-0245-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — miR-542-3p regulated osteogenic transition by targeting BMP7. yVSMCs overexpressing miR-542-3p (miR-yVSMCs) were transduced with BMP7-overexpressing lentivirus or control empty virus. After 7 days of induction by β-GP, the mRNA (a) and protein (b) expression levels of smooth muscle markers (SM22α and calponin) and osteogenic markers (OPN and Runx2) were detected. c Quantification of protein expression levels by densitometric analysis. d Calcium deposition detected by Alizarin red staining. e Quantification of calcium deposition. Control: miR-yVSMCs; Vector: miR-yVSMCs transduced with control empty virus; BMP7 overexpression: miR-yVSMCs transduced with BMP7-overexpressing lentivirus. n = 3. Versus control and vector groups, *p < 0.05, **p < 0.01