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. Author manuscript; available in PMC: 2019 Dec 12.
Published in final edited form as: Stem Cell Res. 2019 Aug 23;40:101550. doi: 10.1016/j.scr.2019.101550

Fig. 4.

Fig. 4.

Loss of Fancd2 increases OXPHOS and mtROS. (A) Increased mitochondrial respiration in Fancd2-KO HSPCs. Oxygen consumption rates (OCR) were measured in BM LSK cells using the Seahorse XF96 analyzer. A representative experiment of three is shown. Basal OCR and Maximal OCR are shown on the right panel. (B) Increased mitochondrial membrane potential, measured using Tetramethylrhodamine ethyl ester perchlorate (TMRE), in Fancd2-KO LSK cells. Results are presented as mean ± SD of three independent experiments. (C) Increased mitochondrial ROS, measured using mitoSOX, in Fancd2-KO LSK cells. Results are presented as mean ± SD of three independent experiments. (D) Decreased lactate production, measured using the Biovision Lactate Assay kit, in Fancd2-KO LSK cells. Results are presented as mean ± SD of three independent experiments. ∗p < .05, ∗∗p < .01.