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. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: Dev Cell. 2019 Feb 28;49(1):130–144.e6. doi: 10.1016/j.devcel.2019.01.027

Figure 1. TBK1 interacts with and phosphorylates Stx17 at Ser-202.

Figure 1.

(A) MS analysis showing comparison of GFP or GFP-Stx17 peptides in co-IPs with TBK1, extracted from Table S1(upper panel). Co-IP between endogenous Stx17 and TBK1 in 293T cells (lower panel). (B) Co-IP of FLAG-Stx17 with Myc-TBK1WT or Myc-TBK1K38D in 293 T cells. * indicates the phospho-shift in FLAG-Stx17 induced by Myc-TBK1WT (lane 2) and not by Myc-TBK1K38D (lane 3). (C) Mass-spec analysis showing phosphorylation of FLAG-Stx17 at Ser-202 residue induced by Myc-TBK1 (right panel) and not by Myc alone (left panel). (D) Western blot analysis of Stx17pS202 levels in WT, Stx17KO and TBK1KO HeLa cells treated with 500 ng/ml of LPS for 4h. White asterisk, Stx17pS202 (note minor levels in TBK1 knockout cells). (E) A schematic showing phosphorylation of Stx17 at Ser-202 residue by TBK1. See also Figure S1 and Table S1.