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. 2019 Dec 2;15(12):e1008209. doi: 10.1371/journal.ppat.1008209

Fig 3. The diameter of labeled rings may differ between glycoproteins and between free and cell-bound viral particles.

Fig 3

The diameters of 2,467 rings observed on raw, unmodified images obtained in gSTED mode were measured and compared according to the type of glycoprotein labeled and whether the particles were bound to cells or not. (A) Purified virions were attached to glass coverslips at room-temperature (Free) or bound to HFFF cells at 4°C. (B) Purified virions were attached to glass coverslips at 4°C (Free) or bound to HeLa cells at 4°C. Statistical differences were measured using an unpaired student’s t-test after the Gaussian distribution of values was verified using a Shapiro-Wilk normality test. ns: p >0.01, ***: p<0.0001 (B). n: number of analyzed particles per condition.