Table 5. Summary of advantages and disadvantages of the selected panel of host cells for Leishmania susceptibility testing.

Cell type	Primary cells			Cell lines
	PEC	BMMφ	PBMC	THP-1	J774	RAW
	Peritoneal macrophages	Myeloid progenitor cell derived	Monocyte derived macrophages	human monocyte derived	BALB/c monocyte derived	BALB/c macrophage derived
Source	Mouse	Mouse	Human blood	Commercial	Commercial	Commercial
Cell stimulation	starch or thioglycollate	L929 supernatant GM-CSF	NA	PMA, VitD3 or RA	NA	NA
Approximate yield	107 cells/mouse	107−108 cells/mouse	105−106/mL (10% monocytes)	NA	NA	NA
Ethical concerns	+++	+++	++	-	-	-
Biological relevance	++	++	+++	-	-	-
Receptivity for infection	+++	+++	+	+	++	++
Intracellular multiplication	+	++	++	+	+/-	+/-
Reproducibility	+++	+++	+/-	+/-	++	++
Cost	++	++	+/-	+/-	+/-	+/-
Consumables needed	+	++	+++	+	+	+
Technical complexity	+	+++	++	+/-	+/-	+/-