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. 2019 Dec 2;15(12):e1008110. doi: 10.1371/journal.ppat.1008110

Fig 2. Targeting of StTCP23 mRNA for silencing by small interfering (si) RNAs derived from PSTVd-RG1.

Fig 2

(A) Schematic diagram of the mRNA encoding StTCP23. Complementarity between a target sequence located in the 3สน UTR and small RNAs derived from the PSTVd-RG1 genome and beginning at nt position 45 (blue line), 46 (red line), or 47 (green line) are shown. The PairFold online tool was used to predict the minimum free energy of the resulting RNA duplexes. Purple line, portion of the StTCP23 coding sequence used for VIGS. (B, C) Effects of PSTVd-RG1 infection on StTCP23 mRNA levels at different times post inoculation. Panel B, northern blot analysis of total leaf RNA extracted at 91 dpi, rRNA was used an internal loading control. Panel C, RT-qPCR analysis of the same series of RNA samples used to monitor PSTVd-RG1 replication (see Fig 1B). (D) Vd-sRNA46 expression profile in different development stage of PSTVd-RG1-infected plants.