Table 1.
Antibodies |
Specificity |
Host |
Dye |
Supplier |
Catalogue No. |
Dilution |
Primary antibodies | CD8 | Rat | Serotec, Oxford, United Kingdom | MCA1768T | 1:500 | |
CD11b | Rat | Serotec | MCA711 | 1:60 | ||
CD11c | Hamster | Abcam | ab33483 | 1:20 | ||
CD45 | Rat | Santa Cruz Biotechnology, Dallas, TX | sc-59071 | 1:20 | ||
GS | Guinea Pig | Synaptic Systems | 367005 | 1:400 | ||
GFAP | Mouse | Sigma Aldrich Corp., St. Louis, MO | G3893 | 1:500 | ||
Iba1 | Guinea Pig | Synaptic Systems | 234 003 | 1:500 | ||
NeuN | Guinea Pig | Synaptic Systems | 266 004 | 1:400 | ||
RPE65 | Mouse | Abcam | ab13826 | 1:50 | ||
TMEM119 | Rabbit | Abcam | ab209064 | 1:80 | ||
TMEM119 | Rabbit | Synaptic Systems | 400 002 | 1:500 | ||
Vimentin | Chicken | Abcam | ab39376 | 1:125 | ||
Secondary antibodies | Chicken | Goat | Texas Red | Life Technologies, Carlsbad, CA | A11008 | 1:100 |
Guinea Pig | Goat | Alexa Fluor 488 | Abcam | ab150185 | 1:400 | |
Hamster | Goat | Texas Red | Abcam | ab5743 | 1:200 | |
Mouse | Goat | Alexa Fluor 594 | Thermo Fisher | A-11032 | 1:600 | |
Mouse | Donkey | Alexa Fluor 647 | Invitrogen, Carlsbad, CA | A31571 | 1:200 | |
Rabbit | Donkey | Alexa Fluor 647 | Invitrogen | A31573 | 1:200 | |
Rat | Goat | Texas Red | Life Technologies | A11007 | 1:200 | |
Rat | Rabbit | Texas Red | Abcam | ab6732 | 1:200 |