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. Author manuscript; available in PMC: 2019 Dec 12.

Published in final edited form as: Methods Enzymol. 2019;626:499–538. doi: 10.1016/bs.mie.2019.08.004

Fig. 4 — Schematic workflow for OxcyscPILOT. Panel (A) represents the cohort of WT/AD brain samples. Panel (B) shows the basic steps which include NEM-blocking (SNO)/reduction (total), tryptic digestion, selective reduction, and enrichment (SNO)/enrichment (total). Panel (C) represents the On-Resin dimethylation, TMT tagging, elution, alkylation, and LC-MS/MS and MS3 analysis.

Fig. 4 — Schematic workflow for OxcyscPILOT. Panel (A) represents the cohort of WT/AD brain samples. Panel (B) shows the basic steps which include NEM-blocking (SNO)/reduction (total), tryptic digestion, selective reduction, and enrichment (SNO)/enrichment (total). Panel (C) represents the On-Resin dimethylation, TMT tagging, elution, alkylation, and LC-MS/MS and MS3 analysis.