FIG 3.

A. niger and F. graminearum deleted for the Δ3(E)-desaturase-encoding gene show reduced AFP susceptibilities. (A) Growth of A. niger wild-type (wt) strain N402 (gray bars) and ΔdtdA strains NP1.15 (black bars) and NP1.16 (white bars) at different AFP concentrations. (B) Growth of F. graminearum wild-type (wt) strain (gray bars) and Δ3(E)-desaturase knockout strain (D3KO; white bars). Growth was evaluated by determining the optical density at 600 nm in microtiter plate cultivations. Data were normalized to the growth, i.e., 100% growth, of control samples not treated with AFP. Data are from two independent experiments, each performed as a technical triplicate. Error bars express standard deviations. P values indicate significance of those values where the differences, with respect to the corresponding error bars, were low.