TABLE 2.
MS/MS analysis of fungal strains expressing or lacking a Δ3(E)-desaturase genea
| Strain | Δ3(E)-desaturase | m/z GlyCer [M + Na+] |
m/z hexose-sphingobase fragment [M + Na+] |
|---|---|---|---|
| P. pastoris BBA21.3 (induced) | Present | 776 (∼15%), 778 (∼85%) | 496, 496 |
| P. pastoris BBA21.3 (not induced) | Absent | 778 | 496 |
| P. pastoris NP7.1 (not induced) | Absent | 778 | 496 |
| P. pastoris NP7.1 (induced) | Absent | 778 | 496 |
| A. niger NP1.15 (ΔdtdA) | Absent | 778 | 496 |
| A. niger N402 (18°C) | Present | 776 | 496 |
| A. niger N402 (30°C) | Present | 776 | 496 |
| A. niger N402 (37°C) | Present | 776 (∼75%), 778 (∼25%) | 496, 496 |
a
m/z, mass-to-charge ratio.