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. 2019 Nov 25;8:e50253. doi: 10.7554/eLife.50253

Figure 2. SRK03 and SRK28 are successfully expressed in A. thaliana and represent phenotypically distinct female recognition specificities.

(A) Fluorescence microscopic observation of pistils from SRK-transformed A. thaliana plants pollinated with S03 and S28 pollen from A. halleri. A robust self-incompatibility reaction is observed between cognate alleles, whereas a compatible reaction is observed between non-cognate alleles. White arrows indicate pollen tubes that germinated in the stigma, and are specific to compatible reactions. Bar = 0.3 mm. (B) Number of germinated pollen grains per stigma after pollination. Two SRK03 and SRK28 lines were pollinated with A. halleri pollen expressing either S03 (blue) or S28 (red) specificities. The number of pollinated pistils for each pollination assay is indicated on the top of the figure. The median value for each cross is represented by a horizontal bar.

Figure 2—source data 1. Number of germinated pollen grains per stigma after pollination.
Two SRK03 and SRK28 lines were pollinated with A. halleri pollen expressing either S03 or S28 specificities.

Figure 2.

Figure 2—figure supplement 1. Localization of SRK expression by GFP florescence microscopy.

Figure 2—figure supplement 1.

(A) Flower of an untransformed plant (no SRK expression). (B,C) Flowers of two SRK03p:GFP transformed lines. (D,E) Flowers of two SRK28p:GFP transformed lines. Promoters of both SRK03 and SRK28 drive GFP expression specifically in stigmatic papillae cells (white arrows). Bar = 1 mm.
Figure 2—figure supplement 2. Pattern of proSRK03 activity along development stages of floral buds.

Figure 2—figure supplement 2.

Floral buds and pistils of SRK03p:GFP-transformed plants are observed under optical (above) and UV light (below), respectively. Developmental stages (Smyth et al., 1990) are indicated between brackets. SRK expression is located between stages 11 and 14, with a strong decrease of GFP fluorescence in mature flowers (old stage 15). Similar results were observed for the SRK28p:GFP lines (data not shown). No GFP signal was observed in any other part of the plant (inflorescence stems, leaves and non-reproductive floral organs, data not shown). Black bar = 1 mm, white bar = 0.5 mm.