Table 1. Comparison of peak protein numbers in actin patches.

Calibration methods: Sirotkin, Arasada, Chen, Epstein, MacQuarrie: a range of internal standards tagged with YFP or GFP; Basu: comparison with actin patches in other cells marked with Arp2/3 complex subunit mYFP-arc5 assumed to be 330 molecules per patch from Sirotkin et al. (2010); Sun: External standard nanoparticle with 120 eGFPs; Galletta: internal Cse4 standard; Picco, Manenschijin: internal Nuf2 standard which was calibrated as a ratio with Cse4.

Protein	S. pombe				S. cerevisiae
	Sirotkin et al. (2010)a; Arasada and Pollard (2011)b; Chen and Pollard (2013)c; Epstein et al. (2018)d	Basu and Chang (2011)	MacQuarrie et al. (2019)	Sun This study	Sun This study	Picco et al. (2015) Rvs167 125	Manenschijn et al. (2019)	Galletta et al. (2012)
Actin	7500 a; 4050 b						3563
Arp2/3 complex subunits	320, 320, 320 a	~150		304 (302)	294	210	247	300
WASp/Las17	230 a; 140 b; 135 c	125	~150	138	102	46
WIP/Vrp1	140 a			95	78
Myosin-I/ Myo3, Myo5	400 a; 170 b; 240 c		~350	170	Myo5: 200 Myo3: 100	Myo5: 132	Myo5: ~130 Myo3: ~60
Fimbrin/Sac6	910 a; 800 c; 1000, 1600 d			675	545		455
End4/Sla2 (HIP1R)	160 a; 70 c			124	133	37, 47	~40
Pan1/End3 (Intersectin)	Pan1: 260a; 160 c			Pan1: 219	Pan1: 131 End3: 100	End3: 60
Sla1					168	91	~90
ABP1					800	423	515	810
Clathrin HC	40 a
Clathrin LC	40, 30 a	~40
Capping protein	230 a						197	200
App1	150 a
Coronin	490 a; 320 b
Twinfillin	210 a
F-BAR Cdc15	125 b		~70
F-BAR Bzz1	90 b
Dip1		20
Bbc1			~50
Amphiphysin						Rvs167 125