Table 3. Peak molecules per patch vs total molecules per cell for various endocytic proteins in budding and fission yeast.
| A. Peak molecules per patch vs total molecules per cell in budding yeast | |||
|---|---|---|---|
|
S. cerevisiae
Protein |
Total molecules per cell | Peak molecules per patch | Ratio |
| Ho et al. (2018) | Sun (This study) | ||
| Arp2/3 complex subunits | 12,784, | 294 | 0.023 |
| WASp/Las17 | 3134 | 102 | 0.033 |
| WIP/Vrp1 | 3559 | 78 | 0.021 |
| Myosin-I/ Myo3 and Myo5 |
Myo5, 6051 Myo3, 4222 |
Myo5:200 Myo3:100 |
0.033 0.024 |
| Fimbrin/Sac6 | 24,543 | 545 | 0.022 |
| HIP1R/Sla2 | 8879 | 133 | 0.015 |
| Intersectin /Pan1 and End3 |
Pan1:9830 End3:6064 |
Pan1: 131 End3:100 |
0.013 0.016 |
| Sla1 | 8776 | 91 | 0.010 |
| Abp1 | 18,301 | 800 | 0.044 |
| B. Peak molecules per patch vs total molecules per cell in fission yeast | |||
|
S. pombe
Protein |
Total molecules per cell | Peak molecules per patch | Ratio |
| Sirotkin et al., 2010; Wu and Pollard, 2005 | Sun (This study) | ||
| Arp2/3 complex subunits | ~40,000 | 304 (302) |
0.008 |
| WASp/Wsp1 | 68,000 | 138 | 0.002 |
| WIP/Vrp1 | 19,000 | 95 | 0.005 |
| Myosin-I | 63,000 | 170 | 0.003 |
| Fimbrin/Fim1 | 87,000 | 675 | 0.008 |
| HIP1R/End4 | 22,000 | 124 | 0.006 |
| Intersectin/Pan1 | 27,000 | 219 | 0.008 |