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. 2019 Dec 6;10:2700. doi: 10.3389/fmicb.2019.02700

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Copyright © 2019 Larke-Mejía, Crombie, Pratscher, McGenity and Murrell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of isoA sequences obtained during this study. Trees were constructed with the neighbor-joining method. The analysis was carried out with 28 complete isoA sequences from extant isoprene-degraders, new isoprene-degrading isolates obtained in this study (filled circles, red for isolates from the willow soil DNA-SIP) and 11 complete isoA metagenome sequences [green triangles, wsMG (from willow soil SIP-metagenome); blue open triangles, plMG (from poplar leaf metagenome in blue from Crombie et al., 2018)]. Following removal of gaps and missing data, there were 1,428 bp in the alignment. Bin numbers are included following the isoA-containing contig identifications. Names of neighboring isoprene metabolic genes in the same contig are included in parentheses. Bootstrap values [1000 replications (Satola et al., 2013)] over 75% are shown as circles in the nodes. The scale bar indicates nucleotide substitutions per site.