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. 2019 Dec 6;7:400. doi: 10.3389/fbioe.2019.00400

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Copyright © 2019 Hong and Do.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological differences in diverse neural differentiation approaches. The differentiation of pluripotent stem cells into a neural lineage was developed in a stepwise manner: 2D, 3D, and brain organoid. A depiction of the morphologies of growing neural stem cells and neural rosettes in 2D monolayer cultures. A 3D neurosphere formed with the floating culture technique. Folded brain organoid structure formation after culture embedding in Matrigel and differentiation of pluripotent stem cells. Layer division of early neurons (Tuj-1 positive) and neural progenitors (Sox2 positive) identified by immunocytochemistry. This figure was modified with permission from Stem Cell Biology, published by Life Science Publishing Co.