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. 2019 Dec 6;10:2870. doi: 10.3389/fimmu.2019.02870

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Copyright © 2019 Li, Wang, Cao, Bao, Li, Sun, Bai, Fu, Ma, Zhang, Li, Chen, Liu, An, Wu, Lu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cattle IgG antibody titers against FMDV in sera, and the virus-specific plasmablast-derived mAbs from 3 infected cattle. (A) A total of 3 cattle (#1217, #1234, and #0005) were sequentially infected with O/Mya/98, O/HN/CHA/93, and O/Tibet/99 strains at days 0, 35, and 132. Sera were pooled at days 0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 132, 169, 223, and 225 after first infection. IgG titres were expressed as the reciprocal log10 of serum dilutions that yielded 50% of the absorbance value of the negative control wells in LPB-ELISA. IgG titres less than the sensitivity of the assay (0.9) were adjusted to 0.6 in the figure. (B) IgG plasmablasts from the 3 cattle were collected to produce mAbs, which were specific for FMDV in 23 out of 26 cells from #2334, 27 out of 37 cells from #1217, and 5 out of 6 cells from #0005.