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. 2019 Dec 6;10:1591. doi: 10.3389/fpls.2019.01591

Figure 5.

Figure 5

Triple response development of Arabidopsis wild-type Col-0 and ethylene insensitive ein2-1 on increasing concentrations of ACC with and without AIB treatment. Arabidopsis wild-type Col-0 and ethylene insensitive mutant ein2-1 were sown in darkness on 0, 0.1, 0.25, 0.5, 0.75, 1, 5, 10, and 50 µM ACC with or without treatment with 2 mM AIB. (A) Ethylene production rates of 4-day-old etiolated Col-0 seedlings (n = 30; 4 independent replicates). (B) Pictures of representative 4-day-old etiolated Col-0 and ein2-1 seedlings. (C) Hypocotyl length and (D) root length of Col-0 seedlings (38 ≤ n ≤ 55; 3 independent replicates). (E) Hypocotyl length and (F) root length of ein2-1 seedlings (22 ≤ n ≤ 42; 3 independent replicates). Different letters indicate statistically significant differences between the different groups (Kruskal-Wallis, Dunn’s Multiple Comparison Test, P < 0.01). Error bars are SD. The relative values of panels (B–E) are depicted in Figure S4 . Effect sizes are presented in Table S1 . P < 0.01 is indicated with *.