Figure 2.

Inhibition of Candida biofilm formation. Compound 1 and amphotericin B were evaluated for their ability to inhibit biofilm formation by (a) C. albicans P60002 and (b) C. auris strain 390. An overnight suspension of C. albicans was diluted in RPMI-1640 medium supplemented with MOPS (to achieve a starting inoculum ~5 × 10⁵ CFU/mL) and exposed to compound 1 or amphotericin B (at the indicated concentrations) or left untreated for 24 hours at 35 °C to permit biofilm formation. The biofilm was stained with 0.1% crystal violet, de-stained with ethanol, and biofilm mass quantified at OD₅₉₅. Data represents percent inhibition of biofilm mass by compound 1 relative to the untreated control. Data were analyzed via an unpaired one-way t-test (P < 0.05). Asterisks (*) indicate statistical difference between amphotericin B-treated and compound 1-treated wells.