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. 2019 Dec 12;9:18919. doi: 10.1038/s41598-019-55364-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proteome changes in SCH-treated neurons. Hippocampal neurons were cultured in the presence of vehicle or SCH (30 μM) for 6 days. Proteins were harvested and identified as described in the Supplementary Methods section. (a) Representative 2-DE images showing differentially expressed protein spots indicated by circles and labeled with numbers, which correspond to those illustrated in Table 1. Red circles indicate downregulated proteins. The pI range is below the gels, and the molecular mass is on the right of the gels. Summary of the protein spots (b). Magnified images of differentially expressed protein spots (c). Intensities of differentially expressed proteins (d). Relative spot intensities were calculated as ratios versus vehicle-treated controls. Asterisks indicate downregulated spots.