Table 1.
List of differentially expressed proteins in primary culture of hippocampal neurons treated with SCH vs vehicle.
| Spot no. | Serial no. | Gi-accession no. | Identified protein | Gene symbol | Mascot score | Observed Mr (kD) /pI | Protein expression ratio (SCH/vehicle) | Cellular/Biological function |
|---|---|---|---|---|---|---|---|---|
| 1406 | 1 | 51948476 | Cytochrome b-c1 complex subunit 1, mitochondrial precursor | Uqcrfs1 | 191 | 53.5/5.57 | 2.7 | Mitochondrial electron transport |
| 1610 | 2 | 488838 | CaBP1 (Calcium binding protein 1) | Cabp1 | 194 | 47.59/4.95 | 1.7 | Inhibits agonist-induced intracellular calcium signaling through regulating L-type calcium channel |
| 1706 | 3 | 165971447 | MAP1B protein (microtubule-associated protein 1b) | Map1b | 250 | 77.792/8.46 | 2.3 | Neuronal development, microtubule polymerization and stabilization. Autophagy. |
| 2209 | 4 | 293342999 | Ankyrin domain family member F isoform X1 | Potef/Actb | 153 | 42.109/5.31 | 2.7 | Neuronal differentiation and axon guidance |
| 3501 | 5 | 40254595 | Dihydropyrimidinase-related protein 2 | Crmp2 | 127 | 62.638/5.95 | 1419 | Axonal growth and guidance |
| 4102 | 6 | 149028159 | Similar to putative ATP-dependent Clp protease proteolytic (clpP) subunit, mitochondrial precursor | Clpp | 100 | 36.527/7.68 | 3.1 | Proteolysis involved in cellular protein catabolic process. Chaperone function. |
| 5611 | 7 | 25742751 | Dihydropyrimidinase-related protein 1 | Crmp1 | 261 | 62.499/6.64 | 2.2 | Cytoskeleton remodeling and axonal guidance |
| 6003 | 8 | 14091667 | LEK1 | cenpf | 83 | 39.504/4.86 | 1.9 | Regulates microtubule function through its interaction with the LIS1 pathway, mainly with NudE |
| 6607 | 9 | 25742568 | Dihydropyrimidinase-related protein 3 | Crmp3 | 242 | 62.327/6.04 | 0.3 | Cytoskeleton remodeling and axonal guidance |
| 6705 | 10 | 84781723 | Heat shock protein 75 kDa, mitochondrial precursor | Trap1 | 139 | 80.639/6.56 | 1182 | Stress responses, intracellular trafficking, and protein folding |
| 6709 | 11 | 170785225 | Chain A, revised structure of the Munc18a-Syntaxin1 complex | Stx1a | 159 | 69.323/6.76 | 1.7 | Synaptic vesicle docking |
| 7002 | 12 | 71121745 | Hnrph1 protein (heterogeneous nuclear ribonucleoprotein H1) | Hnrnph1 | 141 | 20.795/5.20 | 2.2 | Regulation of RNA splicing. Involved in neurogenesis, differentiation and synaptogenesis |
| 7102 | 13 | 8394076 | Proteasome subunit alpha type-6 | Psma6 | 161 | 27.838/6.34 | 2.1 | Proteolysis in a non-lysosomal pathway |
| 7305 | 14 | 6978491 | Aldose reductase | Akr1b7 | 115 | 36.230/6.26 | 2.6 | Xenobiotic metabolism |
| 8005 | 15 | 4504447 | Heterogeneous nuclear ribonucleoproteins A2/B1 isoform A2 | Hnrnpa2b1 | 107 | 36.041/8.67 | 5.1 | Regulation of RNA splicing. Involved in neurogenesis, differentiation and synaptogenesis |
| 8604 | 16 | 12711692 | Dihydropyrimidinase-related protein 5 | Crmp5 | 86 | 62.071/6.60 | 3.7 | Remodeling of the cytoskeleton |
| 9501 | 17 | 189181716 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial precursor | Oxct1 | 141 | 56.624/8.70 | 3.6 | Extrahepatic ketone body catabolism |