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. 2019 Dec 12;9:18988. doi: 10.1038/s41598-019-55426-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of LPS alone and in combination with Esc(1–21) (4 μM for wt-CFBE and 1 μM for F508del-CFBE) or with 1 μM of Esc(1–21)-1c (panel a) and effect of each Esc peptide alone (panel b) on IL-8 production from wt-CFBE (left panels) and F508del-CFBE cells (right panels), 20 h after treatment. Data are presented as the mean of four independent experiments ± SEM. The level of statistical significance between untreated Ctrl and Esc(1–21)/Esc(1–21)-1c treated cells or between other treatment groups are shown as: **P < 0.01; ***P < 0.001; ****P < 0.0001.