Table 3.
Characterization of submandibular salivary glands in female and male Slc4a11+/+ and Slc4a11 −/− mice.
| Female | Male | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| +/+ | n | −/− | n | +/+ | n | −/− | n | ||
| Body weight (g) | CCh† | 19.87 ± 0.11 | 4 | 18.52 ± 0.41 | 5 | 25.06 ± 0.24 | 4 | 28.86 ± 0.30 | 4 |
| IPR + CCh‡ | 20.55 ± 0.23 | 8 | 18.43 ± 0.42 | 9 | 27.92 ± 0.56 | 7 | 25.28 ± 0.50 | 8 | |
| Gland weight (mg) | CCh | 33.43 ± 0.86 | 4 | 32.60 ± 2.45 | 5 | 57.25 ± 3.64 | 4 | 55.13 ± 2.55 | 4 |
| IPR + CCh | 38.20 ± 1.40 | 8 | 38.89 ± 4.11 | 9 | 69.06 ± 4.26 | 7 | 63.15 ± 0.68 | 8 | |
| Na+ concentration (mmol/L) | CCh | 62.98 ± 2.25 | 4 | 89.83 ± 3.32 ** | 5 | 30.32 ± 5.78 | 4 | 66.97 ± 4.76 ** | 4 |
| IPR + CCh | 24.63 ± 3.64 | 8 | 52.95 ± 5.47 ** | 9 | 11.29 ± 3.67 | 7 | 14.40 ± 1.87 | 8 | |
| K+ concentration (mmol/L) | CCh | 41.66 ± 5.38 | 4 | 41.82 ± 1.50 | 5 | 63.72 ± 4.56 | 4 | 45.75 ± 3.42 * | 4 |
| IPR + CCh | 37.80 ± 0.90 | 8 | 29.72 ± 1.21 ** | 9 | 50.21 ± 2.66 | 7 | 40.43 ± 0.92 ** | 8 | |
| Cl− concentration (mmol/L) | CCh | 77.19 ± 5.26 | 4 | 98.64 ± 4.13 * | 5 | 66.04 ± 2.92 | 4 | 81.92 ± 4.81 * | 4 |
| IPR + CCh | 45.64 ± 2.87 | 8 | 69.36 ± 4.79 ** | 9 | 32.60 ± 2.36 | 7 | 34.86 ± 0.79 | 8 | |
| HCO3 − concentration (mmol/L) | CCh | 5.39 ± 0.95 | 4 | 14.56 ± 0.61 ** | 5 | 9.56 ± 0.96 | 4 | 16.22 ± 0.56 ** | 4 |
| IPR + CCh | 8.96 ± 0.90 | 8 | 12.10 ± 1.33 | 9 | 23.62 ± 2.85 | 7 | 24.64 ± 0.26 | 8 | |
| pH | CCh | 7.43 ± 0.03 | 4 | 7.66 ± 0.02 ** | 5 | 7.96 ± 0.06 | 4 | 8.02 ± 0.10 | 4 |
| IPR + CCh | 8.90 ± 0.21 | 8 | 8.89 ± 0.19 | 9 | 9.08 ± 0.22 | 7 | 9.76 ± 0.24 | 8 | |
Values are the means ± SEM, n represents the number of glands used in each experiment. Superscripts: † = 10 min perfusion with cholinergic receptor agonist carbachol (CCh; 0.3 μmol/L), ‡ = 10 min stimulation with 1.0 μmol/L IPR plus 0.3 μmol/L CCh. *P < 0.05 or **P < 0.01 in bold; female Slc4a11+/+ versus female Slc4a11 −/− mice or male Slc4a11+/+ versus male Slc4a11 −/− mice.