Figure 4. Dysfunction of BP180 significantly increase the level of MDSC attracting chemokines in the skin.
A. Inflammatory cytokine array showed that skin∆NC16A mice exhibited a significant increase in the level of various cytokines and chemokines, including MDSC attracting CXCL1 (KC), CXCL2 and CCL2 (MCP1). B-D ELISA assay confirmed increased levels of CXCL1 (B), CXCL2 (C) and CCL2 (D) in ∆NC16A skin compared to controls. E. LPS treatment (5ug/ml) for 4 hr significantly increases the level of CXCL1 in the condition media of ∆NC16A keratinocyte culture compared to the BP180 functional sufficient control keratinocytes. (*p<0.05, Mann–Whitney U test; n>=5 for each group).