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EC50 values for cytotoxicity toward cultured INS-1 cells measured for wild-type h-amylin and des Lys-1 h-amylin from six independent experiments using three different cell viability assays.
| Assay | EC50 ± SEM (μM) | |
|---|---|---|
| WT | des Lys-1 | |
| Alamar Blue | 31 ± 3 | 73 ± 5 |
| CellTiter-Glo 2.0 | 35 ± 5 | 74 ± 5 |
| CellTox Green | 24 ± 4 | 56 ± 9 |
Statistical analysis was completed using Graph Pad Prism 5. All data are presented as mean ± standard error of mean (SEM).
