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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Protein Expr Purif. 2019 Sep 19;165:105501. doi: 10.1016/j.pep.2019.105501

Fig. 1.

Fig. 1.

(A) Online data and OD600 profile from 10 L fermentation induced at oxygen sufficient conditions. (B) Online data and OD600 profile from 10 L fermentation induced at oxygen limiting conditions with constant kLa. (C) Bax specific expression level (mg/g pellets) and total volumetric productivity (mg/L) under oxygen sufficient and limiting conditions. (D) Bax purification through chitin column: lane 1 marker, lane 2 crude lysate, lane 3&4 flow through, lane 5 wash, lane 6–8 eluted Bax fractions 1–4. (E) Bax Q-column elution profile. The main Bax peak elutes at 30% buffer B.