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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Anal Biochem. 1995 May 1;227(1):140–147. doi: 10.1006/abio.1995.1263

FIG. 5.

FIG. 5.

Competitive immunoassay for HSA showing the steady-state fluorescence polarization of Ru-HSA. The Ru-HSA was added to preincubated mixtures of anti-HSA with increasing amounts of free HSA. Error bars represent the standard deviations of three polarization readings. See Fig. 4 for experimental conditions.