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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Anal Biochem. 1995 May 1;227(1):140–147. doi: 10.1006/abio.1995.1263

TABLE 1.

Lifetimes (τ), Rotational Correlation Times (θ), and Polarizations (P) of [Ru(bpy)2(dcbpy)] free,a Labeled to HSA and Bound to Anti-HSA (Ab)

Sample τb (ns) θb(ns) Pc c Pasd

Ru free (air) 374
Ru free (+O2) 226
Ru-HSA (air) 346 58 0.055 0.029
Ru-HSA (+O2) 264
Ru-HSA + Ab
 240 nM 351 101 0.085 0.063
 480 nM 355 129 0.100 0.073
 1920 nM 330 160 0.122 0.089
 2880 nM 357 178 0.124 0.090
a

Ru free refers to Ru(bpy)2(dcbpy).

b

Excitation 360 nm, emission 660 nm with bandpass 10 nm, 20°C, PBS, pH 7.4.

c

Calculated polarization values using Eq. [1] assuming P0 = 0.35 (r0 = 0.26); see Fig. 2, bottom.

d

Measured steady-state polarization at excitation wavelength 485 nm.