Figure 3.

FRMD7 attenuation modifies the complexity of neurons from directly converted human fibroblasts. (a) The induced cells were stained at day 9 with antibodies against the neuronal marker b3‐tubulin (Tuj1). (b) Percentage of Tuj1⁺ cells in all cells (DAPI⁺) at day 9. *p < .05, unpaired, two‐tailed Student's t tests versus Tuj1⁺ in control, respectively. n = 6 wells from three independent experiments for each condition. Average numbers of Tuj1⁺ or DAPI⁺ cells per field under × 10 lenses were plotted. Total neurite length (c) and number of neurites (d) per Tuj‐1⁺ neurons. *p < .05, unpaired, two‐tailed Student's t tests versus control; n = 30–50 Tuj‐1⁺ neurons from three independent experiments for each condition