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Overexpression of IDH1 constructs in U87 cells. Protein expression levels of IDH1 were detected by western blotting using an anti-Flag antibody in U87 empty vector control stable cells, IDH1 Wt-expressing stable cells and IDH1 R132H Mut-expressing stable cells. IDH1, IDH1, isocitrate dehydrogenase 1; Wt, wild-type; Mut, mutant.
