Table IV.
Comparison of the results of ALK FISH, ALK IHC, EGFR PNA clamping and NGS analysis.
| NGS analysis | |||||||
|---|---|---|---|---|---|---|---|
| Sex | Age | ALK FISH | ALK IHC | EGFR PNA clamping | RNA | DNA | |
| Case 1 | M | 63 | Positive (18/50; 36.0%) | Positive | Wild | EML4-ALK | None |
| Case 2 | F | 53 | Positive (8/50; 16.0%) | Negative | Wild | None | EGFR L858R |
| Case 3 | F | 53 | Positive (8/50; 16.0%) | Negative | Wild | None | ERBB2 Ins |
| Case 4 | F | 74 | Positive (11/50; 22.0%) | Positive | Wild | None | None |
| Case 5 | F | 44 | Positive (25/50; 50%) | Positive | Wild | EML4-ALK | None |
| Case 6 | F | 71 | Negative | Negative | 19Del | None | EGFR 19 Del |
| Case 7 | M | 53 | Negative | Negative | Wild | TPM3-NTRK1 | None |
| Case 8 | F | 49 | Negative | Negative | Wild | None | KRAS G12D |
| Case 9 | F | 53 | Negative | Negative | Wild | None | ERBB2 Ins |
| Case 10 | F | 73 | Negative | Positive | Wild | None | None |
In ALK FISH-positive cases, the number and percentage of tumor cells with split signals were presented. ALK FISH, ALK IHC, EGFR PNA clamping and NGS. ALK, anaplastic lymphoma kinase; FISH, fluorescence in situ hybridization; IHC, immunohistochemistry; EGFR, epidermal growth factor receptor; PNA, peptide nuclei acid; NGS, next-generation sequencing.