REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
KZ52	Maruyama et al., 1999; IBT Bioservices	0260-001
1H3	Qiu et al., 2011; David DiLillo	N/A
c13C6	Wilson et al., 2000; David DiLillo	N/A
h13F6	Wilson et al., 2000; IBT Bioservices	0201-022
StrepMAB-Classic	IBA Life Sciences	2-1507-001; RRID:AB_513133
anti-human CD19 eFluor 450	ThermoFisher	48-0198-42; RRID:AB_11220071
anti-human IgD Brilliant Violet 510	BioLegend	348220; RRID: AB_2561945
anti-human CD38 Brilliant Violet 605	BioLegend	303532; RRID: AB_2562915
anti-human CD71 PE	BioLegend	334106; RRID: AB_2201481
anti-human CD3 Alexa 700	BioLegend	344822; RRID:AB_2563420
anti-human CD27 PerCP-Cy5.5	BD Biosciences	560612; RRID:AB_1727457
anti-human CD20 PE-Cy7	BD Biosciences	560735; RRID:AB_1727450
Goat anti-human IgG-PE	Southern Biotech	2040-09; RRID:AB_2795648
F(ab’)2Goat Anti-Human IgG (Fcγ) HRP	Jackson ImmunoResearch	109-036-098
F(ab’)2 Goat anti-human IgM (μ-chain) HRP	Jackson ImmunoResearch	109-036-129
F(ab’)2 Goat anti-human IgA (α-chain) HRP	Jackson ImmunoResearch	109-036-011
Mouse anti human IgG1 Fc-HRP	Southern Biotech	9054-05; RRID:AB_2796627
Mouse anti human IgG2 Fc-HRP	Southern Biotech	9060-05; RRID:AB_2796633
Mouse anti human IgG3 Hinge-HRP	Southern Biotech	9210-05; RRID:AB_2796699
Mouse anti human IgG4 Fc-HRP	Southern Biotech	9200-05; RRID:AB_2796691
Bacterial and Virus Strains
Ebola ΔVP30-RenLuc virus	Halfmann et al., 2008	N/A
Mouse-adapted Ebola virus	Bray et al., 1999	N/A
NEB Stable cells	New England Biolabs	C3040H
Chemicals, Peptides, and Recombinant Proteins
Alexa Fluor 488 Protein Labeling Kit	ThermoFisher	A10235
Alexa Fluor 647 Protein Labeling Kit	ThermoFisher	A20173
Alexa Fluor 405 NHS Ester	ThermoFisher	A30000
Live/Dead Fixable Near-IR Dead Cell Stain Kit	ThermoFisher	L10119
Lightning-Link (R) R-PE Antibody Labeling Kit	Novus Bio	703-0015
Ebola virus glycoprotein ectodomain (GPec)	Fusco et al., 2015	N/A
Ebola virus delta mucin glycoprotein ectodomain (GPΔmuc)	Fusco et al., 2015	N/A
Ebola virus cleaved glycoprotein ectodomain (GPcl)	Fusco et al., 2015	N/A
Ebola virus secreted glycoprotein (sGP)	Fusco et al., 2015	N/A
Ebola matrix protein (VP40)	Bornholdt et al., 2013	N/A
NP N-terminal domain	Kirchdoerfer et al., 2015	N/A
NP C-terminal domain	Kirchdoerfer et al., 2016	N/A
VP35	Kirchdoerfer et al., 2015	N/A
VP30	Kirchdoerfer et al., 2016	N/A
Bundibugyo virus glycoprotein	IBT Bioservices	0505-015
Sudan virus glycoprotein	IBT Bioservices	0502-015
Reston virus glycoprotein	IBT Bioservices	0504-015
Ebola GP 15-mer peptide library	McElroy et al., 2015	N/A
SMCC activated cross-linked APC	Anaspec	AS-72108
MPER peptide (GP630-649-Cys)	Genscript	Custom synthesis
EZ-Link Sulfo-NHS-LC-LC-Biotin	ThermoFisher	A35358
Avidin D-HRP	Vector Labs	A2004
TempliPhi100 kit	GE Healthcare	25-6400-10
Thermolysin	Promega	V4001
Critical Commercial Assays
BLC Human ProcartaPlex Simplex Kit	ThermoFisher	EPX01A-12147-901
Immune Repertoire Capture technology	Atreca	N/A
Deposited Data
IGH repertoire sequencing data from EVD donors	This paper	Bioproject: PRJNA531326
mAb sequences	This paper	Bioproject: PRJNA531326
AbVec6W vector sequences	This paper	Bioproject: PRJNA531326
pNL-GP vector sequence	This paper	Bioproject: PRJNA531326
Experimental Models: Cell Lines
Human: Expi293F	ThermoFisher	Cat # A14527; RRID:CVCL_D615
Human: Jurkat E6-1	ATCC	Cat # TIB152; RRID:CVCL_0367
Monkey: Vero	ATCC	Cat # CRL-1586; RRID:CVCL_0574
Monkey: Vero VP30	Halfmann et al., 2008	N/A
Experimental Models: Organisms/Strains
Mouse: Female BALB/c	Charles River	Strain Code: 28
Oligonucleotides
Multiplex antibody cloning primer set	Smith et al., 2009	N/A
BIOMED2 IGHV framework primers	Langerak et al., 2012	N/A
Isotype-specific primers for IgA, IgM, IgG	Roskin et al., 2015	N/A
Recombinant DNA
psPax2	Addgene	RRID:Addgene_12260
pMD2.G	Addgene	RRID:Addgene_12259
pNL(CMV)EGFP/CMV/WPREΔU3	Ou et al., 2012; Addgene	RRID:Addgene_41790
pNL-sGPC	Kulkarni et al., 2014	N/A
Ebola GP (Makona strain)	Sino Biological	VG40442-UT
AbVec antibody expression vectors	Smith et al., 2009	N/A
pcDNA6.2-GW/EmGFP-miR	ThermoFisher	K493600
Software and Algorithms
The PyMOL Molecular Graphics System, Version 1.8	Schrödinger	N/A
GraphPad Prism 6	GraphPad Software	N/A
IgBLAST	Ye et al., 2013	https://www.ncbi.nlm.nih.gov/igblast/
IMGT V Quest	Brochet et al., 2008	http://www.imgt.org/
FLASH	Magoč and Salzberg, 2011	http://www.cbcb.umd.edu/software/flash/
cd-hit	Fu et al., 2012	http://cd-hit.org/
Skylign	Wheeler et al., 2014	http://skylign.org/
R	R Development Core Team, 2017	https://www.R-project.org/
Other
Maxisorp 96 well ELISA plates	ThermoFisher	44-2404-21
Clona-cell-TCS medium	Stem Cell Technologies	03814