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. 2019 Dec 6;13:537. doi: 10.3389/fncel.2019.00537

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Copyright © 2019 Ma, Zhang, Bai, Jiang, Yan and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activating α7 nicotinic acetylcholine receptor (α7nAChR) in microglia relieved inflammatory response and induced neuroprotection against oxygen-glucose deprivation (OGD) injury in vitro. (A) The levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the supernatants of primary microglia were detected by ELISA 24 h after the reintroduction of oxygen and glucose. The data were expressed as the mean ± SD and were analyzed by one-way ANOVA with Tukey’s post hoc test. The data were pooled from five independent experiments. ^∗p < 0.05, ^∗∗p < 0.01 compared with the OGD group, ^#p < 0.05 compared with the PHA-543,613 + OGD group. (B) The levels of the anti-inflammatory cytokines IL-4 and IL-10 in the supernatants of primary microglia were detected by ELISA 24 h after the reintroduction of oxygen and glucose. The data were expressed as the mean ± SD and were analyzed by one-way ANOVA with Tukey’s post hoc test. The data were pooled from five independent experiments. ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared with the OGD group, ^##p < 0.01, ^###p < 0.001 compared with the PHA-543,613 + OGD group. (C) The cell viability of neurons co-cultured with microglia was detected by cell counting kit-8 (CCK-8) 24 h after the reintroduction of oxygen and glucose. The data were expressed as the mean ± SD and were analyzed by one-way ANOVA with Tukey’s post hoc test. The data were pooled from five independent experiments. ^∗∗p < 0.01 compared with the OGD group, ^#p < 0.05 compared with the PHA-543,613 + OGD group. (D) The injury of neurons co-cultured with microglia was detected by lactate dehydrogenase (LDH) release assay 24 h after the reintroduction of oxygen and glucose. The data were expressed as the mean ± SD and were analyzed by one-way ANOVA with Tukey’s post hoc test. The data were pooled from five independent experiments. ^∗∗∗p < 0.001 compared with the OGD group, ^#p < 0.05 compared with the PHA-543,613 + OGD group.