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. 2019 Nov 25;22:430–440. doi: 10.1016/j.isci.2019.11.037

Figure 2.

Figure 2

Genetic Organization and Investigation of the min Gene Cluster

(A) Genetic organization of the MIN gene cluster; A, adeylation domain; Ox, oxidase domain; T, thiolation domain; TE, thioesterase domain; Tau, tautomerase domain.

(B) Bioassays of the metabolites produced by related recombinants of S. coelicolor M1154. The indicator strain is Bacillus subtilis.

(C) HPLC analysis of the metabolites produced by related recombinants of S. coelicolor M1154. Std, the authentic standard of MIN; pCHW301, the metabolites of the recombinant S. coelicolor M1154 containing pCHW301; ΔminA, the metabolites of the recombinant S. coelicolor M1154 containing pCHW301ΔminA, and other samples are correspondingly assigned; pSET152, the metabolites of the recombinant S. coelicolor M1154 containing pSET152 as negative control. The aliphatic numbers correspond to those in the bioassay plate.

See also Figures S1–S3; Tables 1 and S1–S4.