Table 1.
Target gene | Direction | Primer sequence (5′-3′) | A. Temp. (°C) |
---|---|---|---|
Arg1 | Forward | CTCCAAGCCAAAGTCCTTAGAG | 56 |
Reverse | AGGAGCTGTCATTAGGGACATC | ||
CD68 | Forward | TGTCTGATCTTGCTAGGACCG | 56 |
Reverse | GAGAGTAACGGCCTTTTTGTGA | ||
Chi3l3 | Forward | CAGGTCTGGCAATTCTTCTGAA | 56 |
Reverse | GTCTTGCTCATGTGTGTAAGTGA | ||
CXCL9 | Forward | TCCTTTTGGGCATCATCTTCC | 56 |
Reverse | TTTGTAGTGGATCGTGCCTCG | ||
CXCL10 | Forward | CCAAGTGCTGCCGTCATTTTC | 58 |
Reverse | GGCTCGCAGGGATGATTTCAA | ||
CXCL11 | Forward | GGCTTCCTTATGTTCAAACAGGG | 56 |
Reverse | GCCGTTACTCGGGTAAATTACA | ||
F4/80 | Forward | GAGATTGTGGAAGCATCCGAGAC | 57 |
Reverse | GATGACTGTACCCACATGGCTGA | ||
GAPDH | Forward | CTGGGCTACACTGAGCACC | 58 |
Reverse | AAGTGGTCGTTGAGGGCAATG | ||
IL-10 | Forward | GCTCTTACTGACTGGCATGAG | 56 |
Reverse | CGCAGCTCTAGGAGCATGTG | ||
iNOS | Forward | GGAGTGACGGCAAACATGACT | 56 |
Reverse | TCGATGCACAACTGGGTGAAC | ||
Mrc1 | Forward | CTCTGTTCAGCTATTGGACGC | 58 |
Reverse | CGGAATTTCTGGGATTCAGCTTC | ||
Retnla | Forward | CCAATCCAGCTAACTATCCCTCC | 56 |
Reverse | ACCCAGTAGCAGTCATCCCA |
A. Temp., Annealing temperature