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. 2019 Dec 6;10:2889. doi: 10.3389/fimmu.2019.02889

Figure 6.

Figure 6

Intracellular HSV-2 derived nucleotides are major factors involved in the enhanced HIV infection in DCs. (A) Immature DCs were exposed to TLR2, or TLR3 agonists alone or in combination with HIV for 16 h or exposed to HSV-2 (HSV) for 2 h followed by HIV for 16 h. HIV gag transcript, IL1-β, TNF, and IFN-β expression were evaluated by qPCR. (B) cGAMP was given directly or delivered via DOTAP to the DCs alone or together with HIV for 16 h or the DCs were exposed to HSV for 2 h followed by exposure to HIV for 16 h. HIV gag transcript, IL1-β, TNF, and IFN-β expression were evaluated by PCR. HIV gag transcript data were normalized to HIV set as 1 and TNF, IL1-β, IFN-β data were normalized to mock set as 1. *p < 0.05; **p < 0.005; ***p < 0.0005. N = 5–8.